α2,3-Sialylation regulates the stability of stem cell marker CD133
Identifieur interne : 001629 ( Main/Exploration ); précédent : 001628; suivant : 001630α2,3-Sialylation regulates the stability of stem cell marker CD133
Auteurs : Fengbiao Zhou [République populaire de Chine] ; Chunhong Cui [République populaire de Chine] ; Yuqing Ge [République populaire de Chine] ; Hong Chen [République populaire de Chine] ; Qiuping Li [République populaire de Chine] ; Zhiyuan Yang [République populaire de Chine] ; Guoqiang Wu [République populaire de Chine] ; Shuhui Sun [République populaire de Chine] ; Kangli Chen [République populaire de Chine] ; Jianxin Gu [République populaire de Chine] ; Jianhai Jiang [République populaire de Chine] ; Yuanyan Wei [République populaire de Chine]Source :
- The Journal of Biochemistry [ 0021-924X ] ; 2010.
Abstract
CD133 is widely used as a marker for the isolation and characterization of normal and cancer stem cells. The dynamic alternation of CD133 glycosylation contributes to the isolation of normal and cancer stem cells, and is supposed to be associated with cell differentiation. Although CD133 has been identified as a N-glycosylated protein, the specific glycosylation status of CD133 remain unclear. Here, we found that CD133 could be sialylated in neural stem cells and glioma-initiating cells, and the sialyl residues attach to CD133 N-glycan terminal via α2,3-linkage. Furthermore, desialylation of CD133 by neuraminidase specifically accelerates its degradation in lysosomes-dependent pathway. Taken together, our results characterized CD133 as an α2,3-sialylated glycoprotein and revealed that the sialylation modification contributes to the stability of CD133 protein, providing clues to understanding the function of CD133 molecular and to understanding the utility of glycosylated CD133 epitopes in defining neural stem cells and tumour-initiating cells.
Url:
DOI: 10.1093/jb/mvq062
Affiliations:
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Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Li, Qiuping" sort="Li, Qiuping" uniqKey="Li Q" first="Qiuping" last="Li">Qiuping Li</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Zhiyuan" sort="Yang, Zhiyuan" uniqKey="Yang Z" first="Zhiyuan" last="Yang">Zhiyuan Yang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Guoqiang" sort="Wu, Guoqiang" uniqKey="Wu G" first="Guoqiang" last="Wu">Guoqiang Wu</name><affiliation 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Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Kangli" sort="Chen, Kangli" uniqKey="Chen K" first="Kangli" last="Chen">Kangli Chen</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Gu, Jianxin" sort="Gu, Jianxin" uniqKey="Gu J" first="Jianxin" last="Gu">Jianxin Gu</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Jianhai" sort="Jiang, Jianhai" uniqKey="Jiang J" first="Jianhai" last="Jiang">Jianhai Jiang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Wei, Yuanyan" sort="Wei, Yuanyan" uniqKey="Wei Y" first="Yuanyan" last="Wei">Yuanyan Wei</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Institutes of Biomedical Sciences of Fudan University, Zhongshan Hospital of Fudan University and Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Shanghai Medical College of Fudan University, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>*Yuanyan Wei, PhD, Key Laboratory of Glycoconjuates Research, Ministry of Public Health and Gene Research Center, Shanghai Medical College of Fudan University, Shanghai, 200032, People’s Republic of China, Tel: 86 21 54237801, Fax: 86 21 54237660</wicri:regionArea><wicri:noRegion>Fax: 86 21 54237660</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Biochemistry</title><idno type="ISSN">0021-924X</idno><idno type="eISSN">1756-2651</idno><imprint><publisher>Oxford University Press</publisher><date when="2010-09">2010</date><date type="e-published" when="2010-08-28">2010</date><biblScope unit="vol">148</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="273">273</biblScope><biblScope unit="page" to="280">280</biblScope></imprint><idno type="ISSN">0021-924X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-924X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">CD133 is widely used as a marker for the isolation and characterization of normal and cancer stem cells. The dynamic alternation of CD133 glycosylation contributes to the isolation of normal and cancer stem cells, and is supposed to be associated with cell differentiation. Although CD133 has been identified as a N-glycosylated protein, the specific glycosylation status of CD133 remain unclear. Here, we found that CD133 could be sialylated in neural stem cells and glioma-initiating cells, and the sialyl residues attach to CD133 N-glycan terminal via α2,3-linkage. Furthermore, desialylation of CD133 by neuraminidase specifically accelerates its degradation in lysosomes-dependent pathway. Taken together, our results characterized CD133 as an α2,3-sialylated glycoprotein and revealed that the sialylation modification contributes to the stability of CD133 protein, providing clues to understanding the function of CD133 molecular and to understanding the utility of glycosylated CD133 epitopes in defining neural stem cells and tumour-initiating cells.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Fengbiao" sort="Zhou, Fengbiao" uniqKey="Zhou F" first="Fengbiao" last="Zhou">Fengbiao Zhou</name></noRegion><name sortKey="Chen, Hong" sort="Chen, Hong" uniqKey="Chen H" first="Hong" last="Chen">Hong Chen</name><name sortKey="Chen, Kangli" sort="Chen, Kangli" uniqKey="Chen K" first="Kangli" last="Chen">Kangli Chen</name><name sortKey="Cui, Chunhong" sort="Cui, Chunhong" uniqKey="Cui C" first="Chunhong" last="Cui">Chunhong Cui</name><name sortKey="Ge, Yuqing" sort="Ge, Yuqing" uniqKey="Ge Y" first="Yuqing" last="Ge">Yuqing Ge</name><name sortKey="Gu, Jianxin" sort="Gu, Jianxin" uniqKey="Gu J" first="Jianxin" last="Gu">Jianxin Gu</name><name sortKey="Jiang, Jianhai" sort="Jiang, Jianhai" uniqKey="Jiang J" first="Jianhai" last="Jiang">Jianhai Jiang</name><name sortKey="Li, Qiuping" sort="Li, Qiuping" uniqKey="Li Q" first="Qiuping" last="Li">Qiuping Li</name><name sortKey="Sun, Shuhui" sort="Sun, Shuhui" uniqKey="Sun S" first="Shuhui" last="Sun">Shuhui Sun</name><name sortKey="Wei, Yuanyan" sort="Wei, Yuanyan" uniqKey="Wei Y" first="Yuanyan" last="Wei">Yuanyan Wei</name><name sortKey="Wei, Yuanyan" sort="Wei, Yuanyan" uniqKey="Wei Y" first="Yuanyan" last="Wei">Yuanyan Wei</name><name sortKey="Wei, Yuanyan" sort="Wei, Yuanyan" uniqKey="Wei Y" first="Yuanyan" last="Wei">Yuanyan Wei</name><name sortKey="Wu, Guoqiang" sort="Wu, Guoqiang" uniqKey="Wu G" first="Guoqiang" last="Wu">Guoqiang Wu</name><name sortKey="Yang, Zhiyuan" sort="Yang, Zhiyuan" uniqKey="Yang Z" first="Zhiyuan" last="Yang">Zhiyuan Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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